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The epithelial tight junction proteins, the most apical component of epithelial intracellular junctions, equip IECs with this function, which seals the paracellular space between the cells and tightly restricts the transport of hydrophilic molecules 22,23,24

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After 24 h of treatment, a large part of the cells fluoresced, showing active caspase and triggering cell death, according to the FLICA test, which was used to identify cell apoptosis